Monford Paul Abishek N

The paper identifies two pathways for RNA 3' end processing in the gal operon: Rho-dependent termination and RNase E-mediated cleavage, which generate pre-galE mRNA 3' ends that are stabilized by a hairpin structure.

This study investigates how transcription-translation coupling state affects termination in the E. coli galactose operon, showing that loop length determines terminator usage.

The paper develops a method to quantify Rho-dependent termination (RDT) in vivo using tRNAarg and qRT-PCR, showing RDT efficiencies of 36%, 26%, and 63% for specific gal operon regions.

The paper identifies Rho-dependent termination sites in vivo using synthetic sRNA by leveraging dsRNA cleavage and 3'-end stabilization.