BioengineeringACS Synthetic BiologyPeer-reviewed
Development of a recombineering system for the acetogen Eubacterium limosum with Cas9 counterselection for markerless genome engineering
Patrick A. Sanford, Benjamin M. Woolston
This paper develops a recombineering system for Eubacterium limosum using RecT and Cas9 counterselection to enable markerless, scarless genomic modifications with high efficiency.
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