Benjamin M. Woolston

13C-Metabolic Flux Analysis of Substrate Co-Metabolism in the Methylotrophic Acetogen Eubacterium limosum.

This study uses 13C-MFA to analyze co-metabolism in Eubacterium limosum, finding that glucose cofeeding reduces flux through the Wood-Ljungdahl pathway and that cysteine provides gluconeogenic carbon.

Metabolic engineering of Escherichia coli to modulate hydrogen sulfide levels in the mammalian gut

This paper engineers Escherichia coli to produce or consume hydrogen sulfide in specific gut regions, demonstrating methods to increase and decrease H2S levels in mice.

Development of a Transcriptional Biosensor for Hydrogen Sulfide that Functions under Aerobic and Anaerobic Conditions

This paper develops a transcriptional biosensor in E. coli to measure hydrogen sulfide (H2S) under both aerobic and anaerobic conditions, with a detection range of 50-750 μM.

Exploring C1 substrate cofeeding in Eubacterium limosum with AneVO, a low-cost anaerobic parallel bioreactor platform

This study uses AneVO to show that cofeeding C1 substrates with CO or glucose enhances growth and acetate productivity in E. limosum.

Development of a recombineering system for the acetogen Eubacterium limosum with Cas9 counterselection for markerless genome engineering

This paper develops a recombineering system for Eubacterium limosum using RecT and Cas9 counterselection to enable markerless, scarless genomic modifications with high efficiency.