Yu-Huan Wang

The paper uses live-cell imaging to show that E. coli degradosome components dynamically partition between membrane and cytoplasm, affecting RNA degradation based on translation strength and physiological state.

This study provides strategies to improve diffusion parameter estimation in single-particle tracking by optimizing exposure time, sampling rate, and nonlinear MSD fitting.

The paper uses single-molecule imaging to show how RNase E's membrane-binding motif and C-terminal domain affect its localization, diffusion, and mRNA degradation in E. coli.