Emad Tajkhorshid

One high-order ion-coupling mechanism generates multiple SLC1A stoichiometries

This study identifies a high-order ion-coupling mechanism in EAAT transporters by showing that dEAAT2 can acquire Na+, H+, and K+ dependence through mutations, mimicking human hEAAT2's complex ion coupling.

Yeast PI(3,4,5)P3 controls vacuole fusion through Vam7 and membrane microdomain assembly

This study identifies PI(3,4,5)P3 as a regulator of yeast vacuole fusion, showing it controls vertex microdomain assembly and retains the SNARE Vam7 on membranes.

Substrate biasing in UCHL5 proteoforms

This study identifies substrate biasing in UCHL5 proteoforms, showing N-terminal ubiquitination activates the enzyme toward monoubiquitin substrates and regulates debranching activity.

Role of stereochemistry on electron transport in peptides

This study examines how stereochemistry affects electron transport in peptides through experiments and simulations, showing that stereochemical modifications alter conductance via aromatic interactions or secondary structure effects.

Structure and Mechanism of a Two-component Lanthipeptide Toxin

The paper presents the first high-resolution structure of two interacting lanthipeptides and shows how they disrupt cell membranes.

A Type III secretion system effector evolved to be mechanically labile and initiate unfolding from the N-terminus.

This paper shows that effector proteins are mechanically labile, unfolding from the N-terminus, which facilitates their secretion via the Type III secretion system.

Probing the Role of Membrane in Neutralizing Activity of Antibodies Against Influenza Virus

This study shows that antibodies against influenza virus neutralize by interacting with the viral membrane, and disrupting these interactions reduces their effectiveness.

Impact of anionic lipids on the energy landscape of conformational transition in anion exchanger 1 (AE1)

This study uses cryo-EM and molecular dynamics to analyze conformational transitions in AE1 and shows how PIP2 lipids inhibit transport by stabilizing the outward-facing state.

Voltage Sensor Conformations Induced by LQTS-associated Mutations in hERG Potassium Channels

The paper identifies intermediate voltage-sensor conformations in hERG channels caused by LQTS-associated mutations using FLIM, tmFRET, and MD simulations.

LetA defines a structurally distinct transporter family involved in lipid trafficking

The paper determines the structure of E. coli LetAB, identifies LetA as a novel transporter family, and proposes a mechanism for lipid transport using proton-motive force.

Lipid-mediated mechanism of drug extrusion by a heterodimeric ABC exporter

This study reveals how lipid competition modulates drug affinity in ABC exporters through structural and simulation analyses of BmrCD.

Enabling Atomistic Modeling and Simulation of Complex Curved Cellular Membranes with xMAS Builder

The paper introduces xMAS Builder, a tool that constructs atomistic membrane models for MD simulations using experimental data, and demonstrates its ability to generate stable models with insights into membrane packing density.

Single-molecule imaging reveals the roles of the membrane-binding motif and the C-terminal domain of RNase E in its localization and diffusion in Escherichia coli

The paper uses single-molecule imaging to show how RNase E's membrane-binding motif and C-terminal domain affect its localization, diffusion, and mRNA degradation in E. coli.

Atomistic Characterization of Beta-2-Glycoprotein I Domain V Interaction with Anionic Membranes

This study identifies key regions and binding modes of beta-2-glycoprotein I domain V interacting with anionic membranes, important for antiphospholipid syndrome pathogenesis.

Structure of alpha-synuclein fibrils derived from human Lewy body dementia tissue

This study determines the atomic structure of alpha-synuclein fibrils from Lewy body dementia tissue using a novel amplification method and solid-state NMR.

Structures and membrane interactions of native serotonin transporter in complexes with psychostimulants

This paper isolates and characterizes the serotonin transporter (SERT) from porcine brain, revealing its monomeric state and structural interactions with psychostimulants like methamphetamine and cocaine.

A Spatial Whole-Cell Model for Hepatitis B Viral Infection and Drug Interactions

This paper develops a whole-cell computational model of HBV infection and drug interactions to predict infection pathways and optimal drug dosing.

Ataxia-linked SLC1A3 mutations alter EAAT1 chloride channel activity and glial regulation of CNS function

The paper investigates how mutations in SLC1A3 (hEAAT1) affect chloride channel activity and glutamate transport in glial cells, linking these changes to the pathology of episodic ataxia type 6.

Mechanism of gating and partial agonist action in the glycine receptor

This study investigates the molecular mechanisms of partial agonist action and conformational transitions in the glycine receptor using electrophysiology, MD simulations, and cryo-EM.

Overexpression of a non-muscle RBFOX2 isoform triggers cardiac conduction defects in myotonic dystrophy

This study identifies overexpression of a non-muscle RBFOX2 isoform as a cause of cardiac conduction defects in myotonic dystrophy by inducing arrhythmia and altering ion channel splicing.