BiophysicsbioRxivPreprint
Chemical interactions in polyethylene glycol-induced condensates lead to an anomalous FRET response from a flexible linker-fluorescent protein crowding sensor
Anshuman Mohapatra, Jeanpun Antarasen, Danielle R. Latham…·Lydia Kisley
This study investigates how PEG-induced phase separation affects FRET-based crowding sensors, showing that CrH2 forms puncta while CrD does not, and identifies biophysical mechanisms behind the sensor response.
DOI 10.64898/2026.02.16.706251