Resurrected nitrogenases recapitulate canonical N-isotope biosignatures over two billion years
Holly R. Rucker, Kunmanee Bubphamanee, Derek F. Harris, Kurt O. Konhauser, Lance C. Seefeldt, Roger Buick, Betül Kaçar
The paper resurrects ancient nitrogenase enzymes and shows their nitrogen isotope signatures are similar to modern ones, suggesting evolutionary invariance and supporting the use of N-isotope biosignatures for detecting life.
Nature Communications · Jan 22, 2026Peer-reviewedEvolutionary Biology