Segmental Isotope Labelling of the Prion Protein: Identification of a Key Residue for Copper-Mediated Interdomain Structure
Francesca A. Pavlovici, Kevin Singewald, Samuel Kaplan, Eefei Chen, Glenn L. Millhauser
The paper identifies His176 as a key residue for copper coordination in the prion protein using sortase-mediated ligation and pulsed EPR, supported by AlphaFold 3 simulations.
bioRxiv · May 1, 2025 · PreprintBiophysics