Kevin Singewald

Segmental Isotope Labelling of the Prion Protein: Identification of a Key Residue for Copper-Mediated Interdomain Structure

The paper identifies His176 as a key residue for copper coordination in the prion protein using sortase-mediated ligation and pulsed EPR, supported by AlphaFold 3 simulations.

Identifying the Copper Coordination Environment Between Interacting Neurodegenerative Proteins: A New Approach Using Pulsed EPR with 14N/15N Isotopic Labelling

This paper uses pulsed EPR with 14N/15N isotopic labeling to characterize Cu2+ coordination between PrPC and Aβ, revealing how copper bridges these proteins in a ternary complex.