Optimizing Tissue Lysis and DNA Extraction Protocols to Enhance Bacterial Diversity Profiling in the Drosophila melanogaster Gut Microbiome
Carlos L. Quiñones-Sanchez, Jan L. Bilbao-Del Valle, Miguel A. Urdaneta-Colon, Tasha M. Santiago-Rodriguez, Imilce A. Rodriguez‐Fernandez
The paper compares DNA extraction and lysis methods for Drosophila gut microbiome profiling, finding that pestle homogenization with the Qiagen kit best preserves bacterial diversity.
Scientific Reports · Jul 30, 2026Peer-reviewedMicrobiology