Sample Preparation and Homology-Driven Search Shape the First Holobiont Proteome of the Marine Sponge Viles ophiraphidites (formerly Topsentia ophiraphidites)
Carolaine da Silva de Araujo, Ana Caroline Silas Rodrigues, Gabrielle Rodrigues de Souza, Fábio César Sousa Nogueira, Alessandra Leda Valverde, Magno Junqueira
The paper compares sample preparation workflows and homology-driven search strategies to characterize the proteome of a marine sponge, finding S-Trap with homology layers provides the most comprehensive and consistent results.
bioRxiv · Oct 21, 2026 · PreprintBiochemistry