Cell BiologybioRxivPreprint
Enhanced endogenous gene tagging in human induced pluripotent stem cells via AAV6-mediated donor delivery
Erik A. Ehlers, Kyle N. Klein, Margaret A. Fuqua…·Brock Roberts
This paper develops a method to efficiently insert fluorescent tags into genes not expressed in hiPSCs using AAV6 delivery, optimized CRISPR conditions, and ddPCR/ICE to minimize INDELs and isolate tagged clones.
3 citations