Brock Roberts

Dynamics of ML-based Morphological Features Indicate a Shear Stress-Dependent Bifurcation of hiPSC-Derived Endothelial Cell States

The paper uses ML to analyze morphological dynamics of hiPSC-derived endothelial cells under shear stress, identifying a bifurcation between two stable cell states.

A human induced pluripotent stem (hiPS) cell model for the holistic study of epithelial to mesenchymal transitions (EMTs)

The paper presents a hiPS cell model to study EMTs by comparing 2D and 3D cultures, measuring changes in cell function, markers, organization, and environment.

Beta-Adrenergic Stimulation and MYH7 G256E Mutant Gene Dosage Drive Hypertrophic Cardiomyopathy Phenotype Penetrance

This study investigates factors influencing HCM phenotype penetrance by comparing MYH7 mutations in isogenic hiPSC-CMs and shows that beta-adrenergic stimulation and gene dosage affect disease expression.

YAP dysregulation triggers hypertrophy by CCN2 secretion and TGFβ uptake in human pluripotent stem cell-derived cardiomyocytes

This study shows that YAP overactivation in HCM mutant cardiomyocytes drives hypertrophy via CCN2 secretion and TGFβ uptake.

Enhanced endogenous gene tagging in human induced pluripotent stem cells via AAV6-mediated donor delivery

This paper develops a method to efficiently insert fluorescent tags into genes not expressed in hiPSCs using AAV6 delivery, optimized CRISPR conditions, and ddPCR/ICE to minimize INDELs and isolate tagged clones.

Multi-scale models reveal hypertrophic cardiomyopathy MYH7 G256E mutation drives hypercontractility and elevated mitochondrial respiration

This study investigates the effects of the MYH7 G256E mutation on myosin function across multiple scales, finding increased hypercontractility and mitochondrial respiration.