Synthetic BiologybioRxivPreprint
Artificial initiation codons and engineered initiator tRNAs enable N-terminal noncanonical amino acid incorporation in intact cell-free translation systems
Haruyuki Furukawa, Yasunori Okamoto, Naohiro Terasaka
The paper develops an orthogonal initiation system for efficient N-terminal noncanonical amino acid incorporation in cell-free translation without disrupting the native methionine pathway.
DOI 10.64898/2026.05.24.725928