Katherine Titterton

This study uses Cell Painting and machine learning to identify hepatotoxic botanical constituents and their mechanisms in human hepatocytes.

Cell Painting in primary human hepatocytes exposed to 1,085 compounds detects bioactivity at lower concentrations than cytotoxicity assays and predicts cell-based, but not cell-free, ToxCast endpoints across feature extractors.

The paper shows that cell counting can predict compound bioactivity as well as Cell Painting or gene expression, but Cell Painting adds unique value in protein-target-specific benchmarks.