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BiochemistrybioRxiv

Nitrocellulose redox permanganometry: a simple method for reductive capacity assessment

J. Homolak, I. Kodvanj, A. Babic Perhoc, D. Virag, A. Knezovic, J. Osmanovic Barilar, P. Riederer, M. Salkovic-Petrisic

Abstract

We propose a rapid, simple and robust method for measurement of reductive capacity of liquid and solid biological samples based on potassium permanganate reduction followed by trapping of manganese dioxide precipitate on a nitrocellulose membrane. Moreover, we discuss how nitrocellulose redox permanganometry (NRP) can be used for high-throughput analysis of biological samples and present HistoNRP, its modification used for detailed analysis of reductive capacity spatial distribution in tissue with preserved anatomical relations.

PDFDOI

02 /Specification & Classification

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