Versatile entomopathogenic activity of Purpureocillium takamizusanense against diverse agricultural pests

Abstract

Purpureocillium takamizusanense is an emerging entomopathogenic fungus with significant potential for managing diverse agricultural pests sustainably. This study assesses the broad-spectrum pathogenic activity of P. takamizusanense strain TCTeb01, isolated from naturally infected lychee stink bugs (Tessaratoma papillosa) in Taiwan. The fungus was evaluated against three economically critical agricultural pests: the coffee berry borer (Hypothenemus hampei), southern yellow thrip (Thrips palmi), and lychee stink bug. Pathogenicity assays revealed effective control against all tested pests, showing strong pathogenic capabilities and a clear concentration-dependent efficacy. The fungus demonstrated rapid infection and significant pest mortality, highlighting its viability as a biological control agent. Comprehensive morphological and molecular analyses confirmed the fungal strains identity and documented additional morphological features, expanding the known variability within the species. Overall, the findings underscore the versatility and robust pathogenic potential of P. takamizusanense. Its effectiveness against pests from different insect orders makes it a valuable addition to integrated pest management strategies. Given its environmental compatibility and reduced ecological impact compared to chemical pesticides, P. takamizusanense can represent a sustainable alternative for agriculture, contributing positively to pest management practices and ecosystem health.