bioRxivBioinformaticsPreprint
A high-quality de novo genome assembly of Asian Crested Ibis (Nipponia Nippon) using long-read and Hi-C data
Youngseok Yu, Sung-jin Kim, Changhan YooniD, Jihun BhakiD, Changjae KimiD, Hyebin ParkiD, Younghui KangiD, Yeonkyung KimiD, Yujin LeeiD, Seung-yeon Kang, Yong-un Shin, Jong BhakiD, Sungwon JeoniD
Abstract
We present TtaoRef1, the highest-quality de novo genome assembly of Asian Crested Ibis (Nipponia Nippon) to date consisting of 134 scaffolds with a length of 1.25 Gb and N50 of 101,183,595 bp. This assembly was generated through the utilization of long-read sequencing and Hi-C data. The assessment of assembly quality, conducted via Benchmarking Universal Single-Copy Orthologs (BUSCO), revealed the presence of 96.8% of completely predicted single-copy genes. TtaoRef1 had 18 times longer N50 value than the previous assembly (ASM70822v1), Furthermore, we conducted the annotation of 24,681 protein-coding genes within the newly assembled genome sequences.
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