Conjugation dynamics and persistence of a carbapenem resistance gene blaOXA-72 from Acinetobacter pittii to Acinetobacter baumannii

Abstract

Carbapenem-resistant Acinetobacter (CRA) has been associated with increased morbidity and mortality in clinical settings. In this study, we explored the transfer potential of a mobilizable plasmid-harboring blaOXA-72 gene between Acinetobacter species originating from patient, municipal wastewater, and pig farm wastewater. PCR-based evidence suggested putative transfer of blaOXA-72 from Acinetobacter pittii to Acinetobacter baumannii. In this pair, the apparent frequency of PCR-marker-positive putative transconjugants varied depending on temperature and meropenem supplementation, with higher number observed at 27{degrees}C compared to 17{degrees}C and 37{degrees}C. Likewise, the presence of antibiotic pressure yields to higher apparent conjugation frequency, however this observation was limited to a singled donor-recipient pair. Further, we revealed a phenotypic conversion in terms of meropenem susceptibility and a fitness cost in the putative transconjugants. While whole genome sequencing did not conclusively verify the presence of blaOXA-72 or fully resolved plasmid configuration, Oxford Nanopore read mapping consistently detected the chromosomal strA gene in all isolates. In contrast, only a limited number of reads aligned with blaOXA-72 gene, traC, or the complete plasmid sequences. Comparative analyses further revealed variations in the surface-associated factors and defense systems composition of the recipient strains, which could be considered as barriers in conjugation. Lastly, the persistence of PCR-detectable marker genes in putative transconjugants was variable and generally unstable over a 30-day period. Overall, these findings provide preliminary insights into the factors that may influence horizontal gene transfer and short-term maintenance of blaOXA-72. IMPORTANCECarbapenem-resistant Acinetobacter (CRA) is a serious clinical concern linked to elevated morbidity and mortality. This study shed light on how the carbapenemase gene blaOXA_72 spreads in the environment via horizontal gene transfer, Using isolates of Acinetobacter pittii and Acinetobacter baumannii from municipal wastewater treatment plant, we show that conjugation efficiency depends on temperature and antibiotic exposure. The process also incurs fitness costs, and the transferred gene persists only briefly in recipient cells. These results emphasize the importance of environmental sources as reservoirs for antimicrobial resistance gene exchange. Additionally, variations in surface structures and defense systems may act as potential barriers to conjugation. Overall, the work improves our understanding of AMR movement and supports the need for better integrated surveillance under a One Health approach.