Antibiotic Resistance in Bacteria

The large conjugative plasmid pAB3 integrates into the chromosome of Acinetobacter baumannii ATCC 17978

The paper shows that the plasmid pAB3 integrates into the chromosome of Acinetobacter baumannii ATCC 17978, leading to chromosomal rearrangements and potential phenotypic changes.

Conjugation dynamics and persistence of a carbapenem resistance gene blaOXA-72 from Acinetobacter pittii to Acinetobacter baumannii

This study investigates the transfer and persistence of the blaOXA-72 gene from Acinetobacter pittii to Acinetobacter baumannii, finding that conjugation efficiency depends on temperature and antibiotic exposure, with limited gene persistence.

Molecular Glue-like Degraders of TEM β-Lactamases by Periplasmic Protease DegP

This study develops molecular glue-like degraders that target TEM β-lactamases for degradation by DegP, showing improved synergy with piperacillin against resistant E. coli.

ARG-PASS v2.0 expands global resistome surveillance to reveal previously unrecognized antibiotic resistance genes reaching the human gut

ARG-PASS v2.0 identifies previously unrecognized antibiotic resistance genes in environmental samples and links them to human gut microbiomes through dietary exposure.

Abundance-weighted analysis and deep functional metagenomic selection reveals unrecognized diversity of soil-derived streptothricin acetyltransferases

The paper uses deep functional metagenomics to discover new streptothricin acetyltransferase genes in soil, revealing previously unknown resistance mechanisms.

Expanding the Repertoire of Associations Between Antibiotic Resistance Genes and Putative Mobile Genetic Element Carriers Using Metagenomic Long Reads

This study identifies new antibiotic resistance gene-mobile genetic element associations using metagenomic long reads from diverse environments.

Characterization of a spiramycin-inactivating macrolide esterase

The paper characterizes EstS, a macrolide esterase from E. coli, showing it preferentially inactivates spiramycin and tylosin, explaining resistance phenotypes.

Ciprofloxacin resistance in Klebsiella pneumoniae: phenotype prediction from genotype and global distribution of resistance determinants

The paper develops a rules-based classifier to predict ciprofloxacin resistance in Klebsiella pneumoniae from genotype data and demonstrates its global distribution association with quinolone consumption.

PBP1A and LdtJ support cell envelope homeostasis and impact selection of Colistin-resistance in Acinetobacter baumannii

This study identifies the functional interplay between PBP1A and LdtJ in Acinetobacter baumannii, showing that disrupting their coordination weakens the cell envelope and increases colistin resistance.

Co-occurrence of VIM-positive non-aeruginosa Pseudomonas spp. and Pseudomonas aeruginosa in the hospital: A detailed molecular comparison of blaVIM-2-containing plasmids from environmental and clinical isolates

The paper identifies two closely-related blaVIM-2-containing plasmids in non-aeruginosa Pseudomonas spp. and P. aeruginosa during a hospital outbreak, highlighting their role in carbapenem resistance transmission.

The global β-lactam resistome revealed by comprehensive sequence analysis

The paper maps the global distribution of β-lactamases across environments and microbes, revealing their abundance and potential origins in plant and wastewater ecosystems.

Insights Into The Early Diversification of blaNDM-1 Genetic Contexts Following its Emergence in Acinetobacter species and Spread to Enterobacterales

The paper analyzes early genetic contexts of blaNDM-1 in Acinetobacter and Enterobacterales isolates to understand its spread and resistance mechanisms.

Most metagenomic vanT calls are not vancomycin resistance genes

The paper shows that most metagenomic vanTG gene calls are misannotations of alanine racemases, not vancomycin resistance genes.

Disruption of cAMP Signalling and RNA Regulation Confers Cefiderocol Resistance in Acinetobacter baumannii

This study identifies two mechanisms of cefiderocol resistance in Acinetobacter baumannii: cAMP signalling disruption and Hfq RNA-binding domain truncation, both linked to iron homeostasis and reduced drug activity.

Towards forecasting antimicrobial resistance evolution: concordance of in vitro and in vivo studies

This study compares in vitro and in vivo antimicrobial resistance evolution data to show that experimental data can predict in vivo resistance emergence.

Structure and dynamics of Burkholderia pseudomallei OXA-57, a distinctive low-efficiency class D β-lactamase with carbapenem-hydrolyzing activity

This paper characterizes OXA-57, a class D β-lactamase from Burkholderia pseudomallei, showing its activity against penicillins and cephalosporins, limited carbapenem hydrolysis, and structural insights into its mechanism.

Environment-mediated metabolic constraints shape antimicrobial resistance evolution in Pseudomonas aeruginosa

This study shows how environmental factors like nutrients, pH, and oxygen affect the evolution of antibiotic resistance in Pseudomonas aeruginosa.

Antibiotic inhibition uncouples growth from horizontal gene transfer

This study shows that different antibiotics create distinct plasmid transfer dynamics, revealing drug-specific residual conjugation windows that are decoupled from growth inhibition.

Development of a stable microbial community for the study of plasmid-mediated antimicrobial resistance in a continuous culturing device

This study develops a microbial community to study plasmid-mediated AMR spread, showing plasmids protect the community from antibiotics and that HGT dynamics can be controlled.

Isoindole-benzohydrazide derivative potentiates colistin activity to overcome resistance conferred by MCR-1 variants in Escherichia coli

This study identifies JNAL-042, an isoindole-benzohydrazide derivative that enhances colistin activity against MCR-1-resistant E. coli.

Forecasting next-year change in antimicrobial resistance genes in Klebsiella pneumoniae and Acinetobacter baumannii from genomic surveillance data

The paper develops a model to forecast next-year changes in antimicrobial resistance genes in K. pneumoniae and A. baumannii using genomic surveillance data, showing better performance than baselines for both organisms.

Gfa, a new integron-borne fosfomycin resistance protein family

The paper identifies Gfa, a new fosfomycin resistance protein family found in integrons, and shows it confers resistance across multiple bacterial species.

Global genomic epidemiology of multi-carbapenemase Enterobacterales

This study analyzes 1501 global genomes of multi-carbapenemase Enterobacterales, identifying common allelic combinations, plasmid distributions, and evolutionary mechanisms.

Cross-species convergence of surface polysaccharides shapes the virulence of an atypical carbapenem resistant uropathogenic Escherichia coli clone

The paper identifies a new carbapenem-resistant E. coli clone, ST361, associated with UTI and shows its virulence is linked to surface polysaccharides and mobile genetic elements.

Chromosomal integration of blaCTX-M redefines the evolutionary trajectory of antibiotic resistance in E. coli through lineage-structured expansion

The paper identifies chromosomal integration of blaCTX-M in E. coli, showing lineage-specific prevalence and suggesting it contributes to the spread of antibiotic resistance.

Bacterial Mercury Resistance Reveals a Robust Species-Structured Human Antimicrobial Mobilome

The paper investigates mercury resistance in gut microbiota of children, finding high prevalence of Hg-resistant bacteria and strong taxonomic structuring of resistance genes.

Divergent genotype-phenotype maps determine the capacity for adaptation to antibiotics among Enterobacteria

This study compares the adaptation of E. coli and K. aerogenes to antibiotics, finding that their differing genotype-phenotype maps determine their capacity to evolve resistance.

PanRes: A database of latent and acquired antimicrobial resistance allowing 3D-based protein homology search

PanRes is a curated database of antimicrobial resistance genes that uses structural clustering and HMMs to improve detection of resistance determinants in metagenomes.

Structure-Informed Stress-Testing of the Standard Numbering Scheme for Class B β-Lactamases Using AlphaFold2

The paper uses AlphaFold2 to model class B β-lactamases without experimental structures, tests the BBL numbering scheme's validity, and identifies structural exceptions.

Leveraging genomes and metagenome-assembled genomes unveils novel diversity and transmission between public transport and clinical settings in Acinetobacter

This study combines metagenome-assembled genomes and clinical genomes to reveal Acinetobacter diversity and transmission between public transport and clinical settings, identifying antibiotic resistance genes across multiple species.