Spatiotemporal control of cortical centrin patterning by regionalized Sfi1 family scaffolding proteins in Stentor coeruleus

Abstract

Summary/AbstractPatterning is fundamental to the development and maintenance of organisms, ensuring functional and structural organization. While patterning is well studied at the level of multicellular organisms, even single cells need to undergo morphogenesis and form spatial patterns. Stentor coeruleus is a large ciliate that has been a classical system for studying patterning and morphogenesis due to its distinctive shape and organization of easily visible cortical structures which show a clear localization along the anterior-posterior body axis. The cortex of Stentor is made of two cytoskeletal layers, one composed of microtubules, and one composed of a network of centrin-family EF hand proteins, which form a branched network in the anterior half of the cell and long thick bundles known as myonemes in the posterior half. Sfi1 family proteins scaffold the assembly of centrin filaments throughout the eukaryotes, and function as scaffolding proteins in myonemal contractile systems found in some ciliates. A set of Sfi1 proteins upregulated during regeneration in Stentor were found to have a role in maintaining anterior/posterior differences in centrin patterning. Loss of this distinct anterior-posterior patterning leads to defects in regeneration and contraction. Using RNAi-mediated knockdown of Sfi1 genes, we have found that cells fail to regenerate their oral apparatus, with early expressed Sfi1 genes more critical for oral primordium (assembly of organized basal bodies that become the new oral apparatus) formation than those expressed later. Additionally, different Sfi1 proteins appear to be recruited sequentially to the growing primordium in an order that matches the time at which they are required for progression of regeneration. Knockdown of Sfi1 isoforms that results in reduced myoneme cables impairs contraction such that cells fail to contract in response to a stimulus or do not fully contract. These findings suggest a model in which regionalized differences in patterning of cytoskeletal assemblies can be modulated by regionalized localization of scaffolding proteins.