Wallace F. Marshall

Spatiotemporal control of cortical centrin patterning by regionalized Sfi1 family scaffolding proteins in Stentor coeruleus

This study identifies Sfi1 family proteins as regulators of anterior-posterior centrin patterning in Stentor coeruleus, showing their role in regeneration and contraction through sequential recruitment during development.

A role for the Stentor syntaxin protein in post-wound cell survival

This study identifies a Stentor syntaxin gene essential for post-wounding cell survival, showing syntaxin-deficient cells have reduced survival and osmotic stress susceptibility.

Sol-gel Transition Drives Hyper-fast Mixing in a Giant Cell

This study identifies a novel mixing mechanism in giant amoebae using a sol-gel transition strategy, achieving rapid cytoplasmic mixing.

Single-cell learning in Stentor coeruleus is governed by a fractional-order low-pass filter

This study analyzes habituation in Stentor coeruleus using frequency domain methods and finds a fractional-order low-pass filter behavior not explained by existing models.

Size-dependent nucleus-vacuole interactions in budding yeast demonstrate a role for steric packing in organelle shape and positioning

The paper investigates how vacuole size affects nucleus shape and position in budding yeast, showing that physical crowding and steric packing influence organelle organization.

A centrin-Sfi1 myoneme fishnet powers ultrafast calcium-triggered contraction in the giant ciliate Spirostomum ambiguum

This paper investigates ultrafast contraction in Spirostomum using myonemes, showing centrin-Sfi1 structures drive calcium-triggered contraction without actomyosin or ATP.

Pearling Drives Mitochondrial DNA Nucleoid Distribution

The paper shows that mitochondrial DNA nucleoid distribution is controlled by pearling and cristae structure, revealing a mechanism for nucleoid spacing and segregation.

Combinatorial constraints predict that mitochondrial networks contain a large component

The paper uses extremal graph theory to show that mitochondrial networks are likely to have a large connected component due to combinatorial constraints, suggesting this pattern may be a null model rather than requiring specific biological explanation.

Phylogeny, morphology, and behavior of the new ciliate species Stentor stipatus

The paper describes a new ciliate species, Stentor stipatus, and reports its phylogenetic, morphological, ecological, and behavioral characteristics.

Mitochondrial function regulates cell growth kinetics to actively maintain mitochondrial homeostasis

The paper shows that mitochondrial function influences cell growth rates to maintain mitochondrial volume homeostasis across cell divisions in yeast.

The biophysical mechanism of mitochondrial pearling

This study identifies a biophysical mechanism for mitochondrial pearling, showing it occurs spontaneously in various cell types and is driven by internal pressure or external tension.

Multi-immersion Oblique Plane Microscope (miOPM): A reconfigurable platform for high-resolution Light-Sheet Fluorescence Microscopy.

The paper introduces miOPM, a reconfigurable LSFM platform that enables high-resolution imaging across multiple spatial scales and refractive indices.

Automated segmentation of soft X-ray tomography: native cellular structure with sub-micron resolution at high throughput for whole-cell quantitative imaging in yeast

This paper introduces an auto-segmentation pipeline for soft X-ray tomography to enable high-throughput, precise 3D morphometric analysis of yeast cells and organelles.

A receptor-inactivation model for single-celled habituation in Stentor coeruleus

This paper develops a receptor-inactivation model to explain habituation in Stentor coeruleus, showing it can replicate key habituation features and predict new experimental outcomes.

An Algebraic Approach to Representing Mitochondrial Dynamics

This paper introduces an algebraic framework to model mitochondrial dynamics as groupoids and defines a distance metric for comparing mitochondrial structures based on morphological operations.

The cell biology and genome of Stentor pyriformis, a giant cell that embeds symbiotic algae in a microtubule meshwork

This paper describes the cell biology and genome of Stentor pyriformis, a mixotrophic ciliate, revealing unique features of its endosymbiotic relationship with Chlorella variabilis.

A scoping study of the whole-cell imaging literature: a foundational corpus, potential for data-mining and research synthesis, and a call for standardization of an emerging field

This paper curates a corpus of whole-cell imaging studies, identifies trends and variability in research practices, and calls for standardization in the field.

Characterization of cellular wound resistance in the giant ciliate Stentor coeruleus

This study investigates how Stentor coeruleus resists mechanical stress during transit through a microfluidic constriction, identifying cytoskeletal and cytoplasmic factors involved in wound resistance.

Biosynthesis of linear protein nanoarrays using the flagellar axoneme

The paper demonstrates the biosynthesis of linear protein nanoarrays by fusing proteins to axonemal proteins, resulting in stable, high-loading arrays that can be purified in one step.

Aging induces aberrant state transition kinetics in murine muscle stem cells

This study finds that aged murine muscle stem cells have delayed activation kinetics but retain a youthful activation trajectory, suggesting changes in state transition dynamics contribute to aging.

Deep convolutional and recurrent neural networks for cell motility discrimination and prediction

This paper develops CNN and LSTM models to classify and predict cell motility, achieving performance comparable to hand-engineered features and showing utility in predicting muscle stem cell motility.

The Transcriptional Program of Regeneration in the Giant Single Cell, Stentor coeruleus

This study uses RNAseq to analyze the transcriptional changes in Stentor coeruleus during oral apparatus regeneration, identifying five gene expression waves and linking them to organelle regeneration and ciliogenesis.

A large kinome in a large cell: Stentor coeruleus possesses highly expanded kinase families and novel domain architectures

This study characterizes the kinome of Stentor coeruleus, revealing expanded kinase families and novel domain architectures that may underlie its regeneration capabilities.