V. Hamel

Glutamylation imbalance leads to photoreceptor degeneration

This study shows that glutamylation imbalance in photoreceptor cells leads to structural defects and degeneration, highlighting its role in maintaining outer segment architecture.

A disease-associated PPP2R3C-MAP3K1 phospho-regulatory module controls centrosome function

This study identifies a phospho-regulatory module involving PPP2R3C and MAP3K1 that regulates centrosome function and is linked to congenital syndromes with gonadal dysgenesis.

CCDC15 localizes to the centriole inner scaffold and regulates centriole integrity and ciliogenesis

This study identifies CCDC15 as a centriolar protein that maintains centriole integrity and ciliogenesis by regulating inner scaffold structure and function.

Nanoscopy of organelles and tissues with iterative ultrastructure expansion microscopy (iU-ExM)

This paper introduces iU-ExM, an iterative expansion microscopy technique that achieves single-molecule light microscopy resolution for organelles and tissues.

Ultrastructure Expansion Microscopy reveals the nanoscale cellular architecture of budding and fission yeast

The paper extends ultrastructure expansion microscopy (U-ExM) to budding and fission yeast, enabling nanoscale imaging of cellular structures and revealing conserved spatial organization.

In situ architecture of the ciliary base reveals the stepwise assembly of IFT trains

The paper visualizes the native structure of the ciliary transition zone and the stepwise assembly of IFT trains in Chlamydomonas cells.

SFI1 and centrin form a distal end complex critical for proper centriole architecture and ciliogenesis

This study identifies SFI1 and centrin as a distal end complex essential for centriole architecture and ciliogenesis in human cells.

The connecting cilium inner scaffold provides a structural foundation to maintain photoreceptor integrity

The paper identifies the connecting cilium inner scaffold (CC-IS) as a structural zipper that maintains photoreceptor integrity by linking microtubules, and shows that its loss leads to retinal degeneration.

The evolutionary conserved complex CEP90, FOPNL and OFD1 specifies the future location of centriolar distal appendages, and promotes their assembly

The architecture of the centriole cartwheel-containing region revealed by cryo-electron tomography

This study uses cryo-electron tomography to reveal the architectural diversity of the centriole cartwheel region across four species, showing structural variations in periodicity and organization.

Homogeneous multifocal excitation for high-throughput super-resolution imaging

This paper develops a high-throughput super-resolution imaging technique to enable large-scale, high-resolution imaging of biological structures.

Molecular resolution imaging by post-labeling expansion single molecule localization microscopy (Ex-SMLM)

This paper introduces post-labeling expansion single molecule localization microscopy (Ex-SMLM) to overcome limitations in super-resolution imaging by preserving ultrastructural details and improving labeling efficiency.

Two alveolin network proteins are essential for the subpellicular microtubules assembly and conoid anchoring to the apical pole of mature Toxoplasma gondii

This study identifies AC9 and AC10 as essential alveolin network proteins required for SPMT assembly and conoid anchoring in Toxoplasma gondii, with loss of these proteins leading to conoidless mature parasites.

WDR90 is a centriolar microtubule wall protein important for centriole architecture integrity.

This study identifies WDR90 as a centriolar microtubule wall protein essential for maintaining centriole structure in human and Chlamydomonas cells.

Imaging beyond the super-resolution limits using ultrastructure expansion microscopy (UltraExM)

This paper introduces UltraExM, a method that enables subdiffraction-resolution imaging of preserved ultrastructures using standard optical microscopy, revealing new insights into centriolar structures.