Optics ExpressBiophysicsPeer-reviewed journal article
Phase Contrast Tomography (PCT)
Ying Ma, Wenjing FengiD, Yunze LeiiD, zhanqiang Li, Lan MaiD, Shun ZhouiD, Yefeng ShuiD, Qilong Tan, Chao ZuoiD, Peng GaoiD
Abstract
Phase contrast microscopy has been employed for 2D imaging of thin samples since its invention. Herein, we propose and demonstrate phase contrast tomography (PCT) that incorporates the scanning of LED illumination with quantitative phase contrast microscopy (QPCM) to realize 3D phase imaging of a sample. The proposed PCT is demonstrated 3D imaging of 200-nm polystyrene microspheres (PMs) and sub-organelles inside COS7 cells. The results reveal that the proposed PCT has high spatial resolution and stability without speckle noise, and thence, it has a great potential to be applied to industrial testing and life science research.
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- FWCI
- 3.64
- Field percentile
- top 9%
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