Excretory-secretory products from the parasite Schistocephalus solidus enhance viability and function of threespine stickleback splenocytes

Abstract

Helminths can modulate the immune systems of their hosts through the release of soluble molecules known as excretory-secretory products (ESPs). To better understand the extent and mechanisms behind helminth-mediated immunosuppression, we used an emerging model of host-parasite interactions, threespine stickleback, Gasterosteus aculeatus, and its tapeworm parasite Schistocephalus solidus. We examined the impacts of exposure to ESPs of S. solidus (SsESPs) originating from four Canadian lakes on the viability and function of threespine stickleback splenocytes. We found that 24 hours of exposure to low concentrations of SsESPs significantly increased overall splenocyte viability with the greatest impact on the lymphocyte population. While SsESP exposure did not alter baseline splenocyte respiratory burst activity, SsESP-treated splenic cultures demonstrated significant increases in ROS production in response to phorbol 12-myristate 13-acetate (PMA) stimulation, suggesting that SsESPs may lower the threshold for activation of the respiratory burst. These results in splenocytes contrast with previous studies demonstrating that SsESPs suppress head kidney leukocytes (HKL) viability and function, suggest that S. solidus-derived excretory secretory products may have cell-or tissue-specific immunomodulatory effects, and highlight the importance of studying host-parasite interactions across diverse immune tissues.