Indole acetic acid and lipopeptide-producing endophytic bacteria from Taxus chinesis: toxicity evaluation of metabolic products

Abstract

Endophytes play an important role in plant growth and development. Some one can produce auxins, ACCs, iron carriers, and so on to help plants grow and resist unhealthy growth environments. In addition, they can produce certain antimicrobial substances to resist pests and diseases. Among them, Bacillus is the most common beneficial endophytic bacterium in plants. In this paper, 20 IAA-producing strains were screened from endophytic bacteria isolated from Taxus chinensis var. mairei plant tissues by high-performance liquid-chromatography (HPLC). Based on the 20 IAA-producing strains, LC-TOF-MS technology was used to screen lipopeptide-producing Bacillus sp. As a result, three strains (KLBMPTC01, KLBMPTC10, and KLBMPTC29) of Bacillus-producing lipopeptides with abundant contents and species were obtained. According to the situation of the IAA and lipopeptides produced by these strains, KLBMPTC10 was selected as the experimental strain for later toxicological tests. In an Ames test and oral toxicity experiments in mice, we did not detect mutagenicity and other physiological toxicity. This is hoping to provide a theoretical basis for forest resource protection and biofertiliser production therewith.