bioRxivBiophysicsPreprint
Left-handed DNA-PAINT for improved superresolution imaging in the nucleus.
H. Geertsema, G. Aimola, V. Fabricius, J. P. Fuerste, B. Kaufer, H. Ewers
Abstract
DNA point accumulation in nanoscale topography (DNA-PAINT) advances super-resolution microscopy with superior resolution and multiplexing capabilities. However, cellular DNA may interfere with this single-molecule localization technique based on DNA-DNA hybridization. Here, we introduce left-handed DNA (L-DNA) oligomers that do not hybridize to naturally present R-DNA and demonstrate that L-DNA PAINT has the same specificity and multiplexing capability as R-DNA PAINT, but greatly improves specific visualization of nuclear target molecules.
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