Interactive design and validation of antibody panels using single-cell RNA-seq atlases

Abstract

Single-cell RNA-sequencing holds promise for identifying novel markers of cellular variation for antibody-based technologies. However, antibody panel design is often difficult due to multiple experimental and biological constraints. We introduce Cytomarker, an interactive platform enabling human-in-the-loop design of antibody panels from single-cell transcriptomic data. We use Cytomarker to spatially profile human mammary tissue subpopulations and develop a novel antibody screening approach to validate granular subpopulation predictions across >3.5M cells.