eLifeNeurosciencePeer-reviewed journal article
Three-Dimensional Two-Photon Optogenetics And Imaging Of Neural Circuits In Vivo
Weijian YangiD, Luis Carrillo‐ReidiD, Yuki BandoiD, Darcy S. PeterkaiD, Rafael YusteiD
Abstract
We demonstrate a holographic system for simultaneous three-dimensional (3D) two-photon stimulation and imaging of neural activity in the mouse neocortex in vivo with cellular resolution. Dual two-photon excitation paths are implemented with independent 3D targeting for calcium imaging and precision optogenetics. We validate the usefulness of the microscope by photoactivating local pools of interneurons in awake mice visual cortex in 3D, which suppress the nearby pyramidal neurons response to visual stimuli.
- organism: mouse
- assay: spatial transcriptomics
- research focus: cell type annotation
- research focus: cell-cell communication
- research focus: spatial domain detection
- approach: experimental assay
- Citations
- 225
- FWCI
- 14.08
- Field percentile
- top 1%
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