Interdependency of respiratory metabolism and phenazine-associated physiology in Pseudomonas aeruginosa PA14

Extracellular electron transfer (EET), the reduction of compounds that shuttle electrons to distal oxidants, can support bacterial survival when preferred oxidants are not directly accessible. EET has been shown to contribute to virulence in some pathogenic organisms and is required for current generation in mediator-based fuel cells. In several species, components of the electron transport chain (ETC) have been implicated in electron shuttle reduction, raising the question of how shuttling-based metabolism is integrated with primary routes of metabolic electron flow. The clinically relevant bacterium Pseudomonas aeruginosa can utilize carbon sources (i.e., electron donors) covering a broad range of reducing potentials and possesses a branched ETC that can be modulated to optimize respiratory efficiency. It also produces electron shuttles called phenazines that facilitate intracellular redox balancing, increasing the complexity of its metabolic potential. In this study, we investigated the reciprocal influence of respiratory metabolism and phenazine-associated physiology in Pseudomonas aeruginosa PA14. We found that phenazine production affects respiratory activity and terminal oxidase gene expression, and that carbon source identity influences the mechanisms enabling phenazine reduction. Furthermore, we found that growth in biofilms, a condition for which phenazine metabolism is critical to normal development and redox balancing, dramatically affects the composition of the P. aeruginosa phenazine pool. Together, these findings can aid interpretation of P. aeruginosa behavior during host infection and provide inroads to understanding the crosstalk between primary metabolism and shuttling-based physiology in the diverse bacteria that carry out EET.\n\nIMPORTANCEPseudomonas aeruginosa is a major cause of healthcare-associated infections and long-term lung infections in people with cystic fibrosis. It can use diverse organic compounds as electron donors and possesses multiple enzymes that can transfer electrons from central metabolism to O2. These pathways support a balanced intracellular redox state and the production of cellular energy. Under hypoxic conditions, P. aeruginosa can reduce phenazines, secondary metabolites that also promote redox homeostasis and that contribute to virulence. We asked how these primary and secondary routes of electron flow influence each other. We found that phenazines affect respiratory function, that the roles of respiratory enzymes in phenazine reduction are highly condition-dependent, and that the complement of phenazines produced is strongly affected by growth in assemblages called biofilms. These results provide a more nuanced understanding of P. aeruginosa redox metabolism and may inform strategies for treating persistent infections caused by this bacterium.