PmaI-dependent pH gradients initiate inter- and intraspecies germling fusion in filamentous fungi

Abstract

Somatic cell fusion among spore germlings is a complex process in filamentous fungi that enables the formation of a syncytial unit and facilitates the exchange of genetic material apart from sexual reproduction. In this study, we investigated the molecular mechanisms underlying germling fusion in the maize pathogen Colletotrichum graminicola. Based on secretomes harvested during the germling fusion process of fusion-competent oval and fusion-deficient falcate conidia, we identified pH as a communication signal. We showed that the plasma membrane localized ATPase PmaI is a probable source for pH gradient formation. Our results also suggest that the a-pheromone receptor CgSte3 mediates sensing of acidic pH gradients, activating downstream the Cell Wall Integrity Mitogen Activated Protein Kinase pathway. We propose a new model for germling interactions, in which pH serves as an interaction initiation signal, resulting in the activation of the cell-dialogue. Our findings have implications for our understanding of fungal communication and pathogenicity, and highlight the importance of pH as a signal molecule in germling fusion.