Daniel J. Lew

Regulation of polarity during morphological transitions in Aureobasidium pullulans

This study investigates the regulation of cell polarity during morphological transitions in Aureobasidium pullulans, showing that Rac1 and Cdc42 have distinct roles in different growth modes.

Organelle scaling over a 100-fold cell size range

The paper examines how organelle content scales with cell volume in a fungus with a large size range, finding that some organelles scale with volume while others do not.

Organelle partitioning in the multi-budding yeast Aureobasidium pullulans

The paper studies organelle inheritance in the multi-budding yeast Aureobasidium pullulans, finding that some organelles are evenly distributed while others are variably inherited.

Septins and cytokinesis in the polymorphic fungus Aureobasidium pullulans

This study shows that the fungus Aureobasidium pullulans can position cytokinetic machinery at bud necks without septins, suggesting a novel CAR positioning strategy.

Regulation of the cell wall integrity pathway at the contact site between mating partners in yeast

The paper shows that the Cell Wall Integrity pathway is downregulated at mating contact sites in yeast to allow cell wall degradation, with Fig2 mediating this process.

A genetic strategy to allow detection of F-actin by phalloidin staining in diverse fungi

The paper introduces a genetic mutation that allows phalloidin staining of F-actin in ascomycete fungi by reverting a single amino acid change in actin.

Mechanisms of nuclear segregation in a multinucleate multibudding yeast

This study examines mitotic nuclear segregation in the multibudding yeast Aureobasidium pullulans, revealing distinct mechanisms compared to uninucleate yeasts.

Allocation of resources among multiple daughter cells

The paper investigates how a multi-budding yeast partitions growth equally among its buds, revealing that actin cable networks enable even partitioning without compensatory mechanisms.

Ratiometric signaling produces robust temporal integration for accurate cellular gradient sensing

This study shows ratiometric signaling allows cells to integrate temporal receptor activity for accurate gradient sensing despite noise.

Optimized vectors for genetic engineering of Aureobasidium pullulans

The paper designs and tests 25 optimized vectors for genetic engineering of Aureobasidium pullulans, enabling dual protein expression and homology-based gene modification.

Negative feedback equalizes polarity sites in a multi-budding yeast

The paper identifies Pak1 as a key protein mediating negative feedback in yeast to enable multiple polarity sites and budding.

Particle-based simulations reveal two positive feedback loops allow relocation and stabilization of the polarity site during yeast mating

A novel stochastic simulation approach enables exploration of mechanisms for regulating polarity site movement

The paper introduces a novel stochastic simulation approach to study how molecular noise regulates polarity cluster movement in yeast cells, finding that cluster mobility depends on molecular distribution and dynamics rather than total abundance.

How yeast cells find their mates

This study shows yeast cells detect pheromone gradients through two steps: initial biased orientation based on receptor occupancy and error correction via partner communication.

A role for Gic1 and Gic2 in Cdc42 polarization

This study identifies Gic1 and Gic2 as regulators of Cdc42 polarization in yeast, showing they function both upstream and downstream of Cdc42 in establishing cell polarity.