The lysogenization of the non-O157 Escherichia coli strains by stx-converting bacteriophage phi24B is associated with the O antigen loss and reduced fitness

Abstract

The ability of the Shiga-toxigenic E. coli (STEC) to produce the toxin depends on the lysogenic conversion by stx-bacteriophages. The canonical stx-phage phi24B can lysogenize a wide variety of E. coli strains. In vivo the secondary lysogenization of symbiotic E. coli strains by the phages released by infecting STEC populations may contribute to the overall patient toxic load and to lead to the emergence of new pathogenic STEC strains. However, in our experiment all the phi24B lysogens obtained from the environmental E. coli isolates had compromised O-antigen (Oag) biosynthesis. These lysogenic strains gained the sensitivity to the T5-like bacteriophages and featured increased sensitivity to the bactericidal activity of the horse serum. We conclude that in most of E. coli strains the Oag effectively restricts phi24B infection. The lysogenic clones predominantly rise from the Oag deficient mutants and therefore they have reduced fitness compared to the parental strain.