Preparation and optimization of MEPEG-PLGA nanoparticles for gene delivery

Abstract

This paper demonstrates a method to prepare a cationic methoxy-terminated polyethylene glycol-polylactic acid polyglycolic acid block polymer (MePEG-PLGA) nanoparticle by a nanoparticle precipitation method was established. This study used single factor design and orthogonal experiment to select the optimal experimental scheme and examined the physical properties of the nanoparticles such as surface morphology, particle size distribution, zeta potential, DNA binding rate, and DNA protection ability. Results indicate that the optimal size of the prepared nanoparticles was 89.7 nm and the surface potential was 28.3 mV. The nanoparticles were scattered under transmission electron microscope, the size was uniform, the surface was smooth, and the distribution was spherical. The DNA binding rate was 80.2 %, and can well protect the contained genes from nuclease degradation. Conclusion The cationic nanoparticles prepared by nanoparticle precipitation method are expected to be highly efficient gene carriers. The preparation of cationic methoxy-terminated polyethylene glycol-polylactic acid polyglycolic acid block polymer (MePEG-PLGA) by nanoparticle precipitation method. Nanoparticle method. Method This study used single factor design and orthogonal experiment to select the optimal experimental scheme, and examined the physical properties of the nanoparticles such as surface morphology, particle size distribution, Zeta potential, DNA binding rate, and DNA protection ability. Results The optimal size of the prepared nanoparticles was 89.7 nm and the surface potential was 28.3 mV. The nanoparticles were scattered under transmission electron microscope, the size was uniform, the surface was smooth, and the distribution was spherical. The DNA binding rate was 80.2 %, and can well protect the contained genes from nuclease degradation. Conclusion The cationic nanoparticles prepared by nanoparticle precipitation are expected to be efficient gene carriers.