Evaluating bacteriophage impact on gut microbiome composition in broad-nosed pipefish (Syngnathus typhle)

Bacteriophages play a crucial role in shaping microbial community dynamics in marine systems and have the potential to stimulate surges in pathogenic bacteria facilitating disease outbreaks. Notwithstanding, bacteriophages also serve as valuable biocontrol agents, underscoring their huge potential for aquaculture therapy treatments. Insights into the intricate tripartite interplay upon an infection with a virulent bacterium, its specific phages and the eukaryotic microbiome could improve our understanding of how bacteria-phage interactions behave in a natural microbial system. This investigation assessed the influence of a virulent Vibrio alginolyticus (K01M1) infection, in tandem with lytic ([fe]St2) and filamentous ([fe]K04M1) Vibrio-specific phages, on the broad-nosed pipefish (Syngnathus typhle) gut microbiome using 16S rRNA amplicon sequencing and non-intrusive gastric swabbing. Microbial communities and diversity metrics were not permanently impacted by Vibrio and phage introductions, while the different infection regimes shaped Vibrio-specific dynamics. In the filamentous phage and Vibrio-only treatments, V. alginolyticus abundances spiked 12 hours post-ingestion. In contrast, V. alginolyticus numbers in the lytic phage and control treatment were significantly reduced, suggesting phage activity and specific elimination of the introduced bacteria. Assisted by relative true-gut tissue samples, a newly implemented non-intrusive swabbing method was successful at discerning the activity of two contrasting phages and supports previous work that encourages the use of [fe]St2 in bacteriophage treatments. Identifying Vibrio-specific phages with similar positive characteristics could be beneficial for the aquaculture trade, which is currently heavily impacted by the antibiotic crisis.