Omega 3 fish oil suppress radiation induced hepato and renal toxicity in mice through modulation in Wnt canonical pathway combined with NHEJ and Intrinsic Apoptotic pathway

Abstract

Radiation is associated with inflammation and oxidative stress, the latter of which contributes to activation of DNA damage and apoptosis. Omega 3 polyunsaturated fatty acids (PUFAs) have been reported to limit oxidative stress and DNA damage. The aim of this study was to evaluate the effect of the Omega 3 PUFA on antioxidant defence in male physiology on mice model. Liver and kidney tissues were obtained from whole body irradiated mice divided under 9 groups (Weight-10mg, 6-8 months old, n=5) and age-matched male controls (6-8 months old, n=5). 6 groups have been orally intubated with (50, 100 and 150) mg/kg BW with Omega 3 fish oil 1hr prior to the radiation exposure. Liver and kidney were surgically obtained after 24 hours and 30 days of radiation exposure. Omega 3 fish oil supplementation increased the level of mRNA expression of Lef1, Axin2, Survivin, Ku70, SOD1, SOD2, Cat, iNOS and decresed the level of Bax and Bcl2 in irradiated with omega 3 fish oil supplementation compare to irradiated alone. Omega 3 fish oil increased SOD scavenging, Catalase, Nitric oxide scavenging activity, Total antioxidant capacity and decrease the lipid peroxidation. The improvements in mRNA level of candidate genes of Wnt canonical pathway, NHEJ pathway, oxidative stress status serve as a stimulus for further investigation of Omega 3 fish oil as supplementation for patients undergo radiation therapy.