bioRxivBiophysicsPreprint
Two-dye-imager DNA-PAINT enables volumetric nanoscopy of expanded cells
Janna EiltsiD, Julia WeingartiD, Cerridwen Kiesel, Harsha Perozhy, Philip KollmannsbergeriD, Arindam Ghosh, Dominic A. HelmerichiD, Markus SaueriD
Abstract
Refined single-molecule localization microscopy methods demonstrated superior localization precisions on isolated sample but remain limited by labeling density and imaging speed in cells. Here we combine expansion microscopy (ExM) with two-dye-imager (TDI)-DNA-PAINT to resolve fine molecular details of protein assemblies in [~]8-fold expanded cells with nanometer resolution. Using lattice light-sheet (LLS) microscopy, Ex-TDI-DNA-PAINT provides a robust platform for three-dimensional (3D) volumetric nanoscopy of the molecular organization of cells.
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