bioRxivBiophysicsPreprint
Nanoscale imaging of expanded cells and proteins with spontaneously blinking dyes
Danush Taban, Marvin JungblutiD, Made BudiartaiD, Dominic A. HelmerichiD, Cerridwen Kiesel, Sarah E. PlutkisiD, Luke D. LavisiD, Donatus KrahiD, Ali ShaibiD, Sören DooseiD, Philip KollmannsbergeriD, Silvio O. RizzoliiD, Gerti BeliuiD, Markus SaueriD
Abstract
Expansion microscopy (ExM) enables nanoscale imaging on standard microscopes, but combining ExM with single-molecule localization microscopy (SMLM) remains difficult, owing to the incompatibility of expanded hydrogels with photoswitching buffers. Here, we introduce a single-step expansion microscopy method that allows SMLM with spontaneously blinking dyes in 6-14x expanded samples, without re-embedding. We demonstrate nanometer-resolution imaging by resolving the organization of the nuclear pore complex (NPC) and the molecular structure of recombinant homotrimeric proliferating cell nuclear antigen (PCNA).
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