C. F. Kaminski

Molecular mechanisms of liposome interactions with bacterial envelopes

The paper investigates how fusogenic liposomes interact with Gram-negative and Gram-positive bacterial envelopes using microscopy, revealing differences in fusion and delivery mechanisms.

Multiparametric sensing of outer membrane vesicle-derived supported lipid bilayers demonstrates the specificity of bacteriophage interactions

This study demonstrates the use of supported lipid bilayers derived from outer membrane vesicles to monitor specific bacteriophage interactions with bacterial membranes.

Intracellular FUS protein accumulation leads to cytoskeletal, organelle and cellular homeostasis perturbations

This study investigates how mutant FUS protein accumulation affects cytoskeletal, organelle, and cellular homeostasis, linking it to cellular aging and potential senescence mechanisms.

A method for the fast and photon-efficient analysis of time-domain fluorescence lifetime image data over large dynamic ranges

The paper improves the center-of-mass method for fluorescence lifetime imaging by enhancing photon efficiency and robustness to background noise across large dynamic ranges.

ERnet: a tool for the semantic segmentation and quantitative analysis of endoplasmic reticulum topology for video-rate super-resolution imaging

ERnet quantifies ER topology dynamics using video-rate super-resolution imaging and semantic segmentation, enabling analysis of ER morphology changes in response to genetic or metabolic perturbations.

Intracellular Aβ42 aggregation leads to cellular thermogenesis

This paper shows that Aβ42 aggregation in live cells increases intracellular temperature, which can be mitigated by aggregation inhibitors and is independent of mitochondrial damage.

Single-molecule sizing through nano-cavity confinement

This paper introduces a method called nano-cavity diffusional sizing (NDS) to measure the size of nanoscale particles and single biomolecules using residence times in nano-cavities.

2-photon-fabricated nano-fluidic traps for extended detection of single macromolecules and colloids in solution

This paper introduces nano-fluidic traps that increase detection times for single macromolecules and colloids in solution by providing local steric confinement.

Correlative microscopy reveals the nanoscale morphology of E. coli-derived supported lipid bilayers

The paper uses correlative AFM and SIM to characterize E. coli-derived supported lipid bilayers at the nanoscale, revealing their composition and interactions with membrane-binding proteins.

Near-native state imaging by cryo-soft-X-ray tomography reveals remodelling of multiple cellular organelles during HSV-1 infection

This study uses cryo-soft-X-ray tomography to image HSV-1 infected cells in near-native state, revealing morphological changes in organelles like mitochondria and cytoplasmic vesicles.

High-throughput super-resolution single particle trajectory analysis reconstructs organelle dynamics and membrane re-organization

This paper introduces a computational method to analyze high-throughput super-resolution single-particle trajectories, enabling reconstruction of organelle dynamics and membrane reorganization.

The SARS-CoV-2 nucleocapsid protein associates with the replication organelles before viral assembly at the Golgi/ERGIC and lysosome-mediated egress

This study tracks SARS-CoV-2 structural proteins' spatiotemporal dynamics during infection, revealing nucleocapsid's early association with ER membranes and lysosome involvement in viral egress.

Distinct domain requirements for EAP45 in HIV budding, late endosomal recruitment, and cytokinesis

This study identifies distinct domain requirements of EAP45 in HIV budding, late endosomal recruitment, and cytokinesis using fluorescent labeling and microscopy.

Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats

The paper creates and tests chimeric BRC repeats to improve RAD51 binding, finding that shuffled modules can enhance binding affinity and function in human cells.

Illuminating chromatin compaction in live cells and fixed tissues using SiR-DNA fluorescence lifetime

This paper develops a method to measure chromatin compaction in live and fixed cells using SiR-DNA fluorescence lifetime, revealing compaction in differentiating cells and decompaction during stem cell transitions.

Contextual flexibility in Pseudomonas aeruginosa central carbon metabolism during growth in single carbon sources

This study investigates how Pseudomonas aeruginosa adapts its central carbon metabolism to different carbon sources, revealing distinct metabolic flux patterns and gene expression changes.

Combining sample expansion and light sheet microscopy for the volumetric imaging of virus-infected cells with optical super-resolution

This paper combines sample expansion with light sheet microscopy to achieve high-resolution 3D imaging of virus-infected cells, revealing new details of the infection cycle.

The structure and global distribution of the endoplasmic reticulum network is actively regulated by lysosomes

This study shows lysosomes actively regulate ER remodeling by repositioning, affecting ER tubule distribution and morphology in response to metabolic changes.

Spatiotemporal analysis of host cell modification during herpes simplex virus 1 replication

This study uses a recombinant reporter virus to analyze the spatiotemporal dynamics of host cell modifications during herpes simplex virus 1 replication.

DNA origami nanostructures as a tool for the targeted destruction of bacteria

This study uses DNA origami nanostructures functionalized with aptamers to deliver lysozyme specifically to bacteria, showing improved efficacy against Gram-positive and Gram-negative targets.

OptiJ: Open-source optical projection tomography of large organ samples

OptiJ is an open-source OPT system for 3D imaging of large transparent samples, offering low-cost, modular hardware and software with improved reconstruction capabilities.

Fast fluorescence lifetime imaging reveals the aggregation processes of α-synuclein and polyglutamine in aging Caenorhabditis elegans

This study uses fast TG-FLIM to analyze α-synuclein and polyglutamine aggregation in aging C. elegans, revealing differences in their aggregation kinetics and amyloid-like features.

Intrinsically aggregation-prone proteins form amyloid-like aggregates and contribute to tissue aging in C. elegans

This study shows that age-dependent protein aggregation in C. elegans forms amyloid-like structures and accelerates tissue functional decline, suggesting a role for protein aggregation in aging.

Structured illumination microscopy combined with machine learning enables the high throughput analysis and classification of virus structure

The paper combines structured illumination microscopy with machine learning to analyze and classify virus structures efficiently.

TriPer, an optical probe tuned to the endoplasmic reticulum, tracks H2O2 consumption by glutathione

The paper develops TriPer, an optical probe that detects H2O2 in the ER, revealing glutathione's role in H2O2 turnover during protein folding.