Comprehensive Network Analysis of miRNA Biogenesis Proteins and Their Ligand Interaction Sites to Identify Novel Targets for Cancer Therapeutics

A network analysis of canonical microRNA (miRNA) biogenesis identified DROSHA, Exportin-5, and DICER1 as essential proteins for both precursor and mature miRNA processing. The analysis revealed strong interactions between these proteins and others involved in miRNA biogenesis, suggesting a complex regulatory network. Ligand binding sites on these key proteins were identified, suggesting potential targets for therapeutic intervention. Our findings indicate that modulating miRNA biogenesis through these proteins could influence cellular protein production and function, providing a promising avenue for developing advanced therapy medicinal products (ATMPs) to impact protein expression in diseases such as cancer.