Single-cell transcriptomics of dynamic cell behaviors

Abstract

Despite advances in spatial transcriptomics, the molecular profiling of dynamic behaviors of cells in their native environment remains a major challenge. We present a method, termed behavioral transcriptomics, that allows us to couple physiological behaviors of single cells in an intact tissue to deep molecular profiling of individual cells. This method enabled us to establish a novel molecular signature for a striking migratory cellular behavior following tissue injury.