A desert endophyte, Priestia megaterium SI1-IITJ, improves fluoride stress tolerance by reducing fluoride content of plant tissues and perturbing salt tolerance and defense genes of Arabidopsis thaliana

Abstract

We isolated a fluoride (F-)-resistant bacterium, Priestia megaterium SI1-IITJ, from thse internal root tissues of several Thar Desert plants, Aerva javanica, Cyperus conglomeratus, Senna tora, and Tephrosia purpurea, tolerating up to 100 mM NaF. The root endophytic behavior of the isolate was confirmed by scanning electron microscopy. SI1-IITJ possesses plant growth-promoting properties, including auxin production (19.8 g mL-1), phosphate solubilization (index 3.64), ACC deaminase (0.54 mmol -ketobutyrate mL-1) and nitrate reductase (0.65 mol mL-1 nitrite) activities, revealed by biochemical tests and whole genome sequencing. SI1-IITJ extrudes F- from the cell, possibly through an F- efflux transporter, CrcB, identified in its genome. Significant growth improvements were observed in Arabidopsis thaliana under F- stress in hydroponics and soil culture upon coculture with SI1-IITJ, which improved the chlorophyll content by 1.6%, total nitrogen by 30.4%, and reduced reactive oxygen species by 48.9% and F- content by 63.9% in plant tissues. A differential gene expression analysis of A. thaliana by transcriptome sequencing indicated an unperturbed F- exporter, AtFEX1, but up-regulation of 55 genes regulating root meristem growth, cell wall modification, chlorophyll biosynthesis, Fe homeostasis, and high salt- and abiotic stress-responsive genes. On the other hand, 103 genes were down-regulated, suppressing systemic acquired resistance, plant defense, and H2O2 production. In conclusion, our results provide genomic insights into the mechanisms of F- toxicity alleviation and plant growth enhancement by a desert PGPR, highlighting Priestia megaterium SI1-IITJ as a potential biofertilizer for mitigating F- stress in plants.