Nature CommunicationsBiophysicsPeer-reviewed journal article
The smfBox: an open-source platform for single-molecule FRET
B. Ambrose, J. Baxter, J. Cully, M. Willmott, E. Steele, B. C. Bateman, M. L. Martin-Fernandez, A. Cadby, J. Shewring, M. Aaldering, T. D. Craggs
Abstract
Single-molecule Forster Resonance Energy Transfer (smFRET) is a powerful technique capable of resolving both relative and absolute distances within and between structurally dynamic biomolecules. High instrument costs, and a lack of open-source hardware and acquisition software have limited smFRETs broad application by non-specialists. Here, we present the smfBox, a cost-effective confocal smFRET platform, providing detailed build instructions, open-source acquisition software, and full validation, thereby democratising smFRET for the wider scientific community.
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- FRETBursts: An Open Source Toolkit for Analysis of Freely-Diffusing Single-Molecule FRET
Antonino Ingargiola, Eitan Lerner, … Xavier Michalet·bioRxiv·Jul 8, 2016
- 48-spot single-molecule FRET setup with periodic acceptor excitation
A. Ingargiola, M. Segal, … X. Michalet·bioRxiv·Oct 26, 2017
- High-throughput smFRET analysis of freely diffusing nucleic acid molecules and associated proteins
M. Segal, A. Ingargiola, … X. Michalet·bioRxiv·Jun 25, 2019
- An Open-source, Cost-efficient Excitation Module for Single-molecule Microscopy
D. R. George, B. Ambrose, … T. D. Craggs·bioRxiv·Feb 28, 2022
- Farewell to single-well: An automated single-molecule FRET platform for high-content, multiwell plate screening of biomolecular conformations and dynamics
A. Hartmann, K. Sreenivasa, … M. Schlierf·bioRxiv·Feb 28, 2023
- ABEL-FRET: tether-free single-molecule FRET with hydrodynamic profiling
H. S. Wilson, Q. Wang·bioRxiv·Sep 29, 2019