X. Michalet

in vivo quantitative FRET small animal imaging: intensity versus lifetime-based FRET

This paper compares intensity-based and fluorescence lifetime-based FRET methods for in vivo small animal imaging, showing that lifetime-based FRET is more efficient and accurate for quantifying drug-target engagement.

In vitro and in vivo NIR Fluorescence Lifetime Imaging with a time-gated SPAD camera

This study demonstrates the use of a time-gated SPAD camera for in vitro and in vivo NIR fluorescence lifetime imaging, showing its effectiveness in monitoring molecular events in tumor xenografts.

Wide-field time-gated SPAD imager for phasor-based FLIM applications

The paper evaluates a wide-field time-gated SPAD imager for phasor-based FLIM, showing it functions as an ideal shot noise limited sensor capable of video rate measurements.

Single Photon, Time-Gated, Phasor-based Fluorescence Lifetime Imaging Through Highly Scattering Medium

This paper develops a time-gated single-photon fluorescence lifetime imaging method to overcome scattering and autofluorescence in vivo.

High-throughput smFRET analysis of freely diffusing nucleic acid molecules and associated proteins

This paper introduces a high-throughput smFRET method using multispot excitation and SPAD arrays to rapidly analyze nucleic acid and protein conformations and interactions.

In vitro and in vivo phasor analysis of stoichiometry and pharmacokinetics using short lifetime near-infrared dyes and time-gated imaging

The paper introduces a method using phasor analysis and NIR dyes to quantify fluorescence lifetime and stoichiometry in vitro and in vivo, demonstrating its application to FRET and pharmacokinetics studies.

Optical crosstalk in SPAD arrays for high-throughput single-molecule fluorescence spectroscopy

The paper examines optical crosstalk in SPAD arrays used for high-throughput single-molecule fluorescence spectroscopy and finds it negligible and undetectable in data analysis.

48-spot single-molecule FRET setup with periodic acceptor excitation

This paper introduces a high-throughput smFRET system using 48 excitation spots and SPAD detectors, enabling rapid subpopulation identification and real-time kinetics studies.

Multispot single-molecule FRET: high-throughput analysis of freely diffusing molecules

The paper introduces a multispot confocal setup for high-throughput smFRET assays and validates its performance using bacterial RNA transcription kinetics.