bioRxivMicrobiologyPreprint
In vitro characterization of multidrug-resistant influenza A(H1N1)pdm09 viruses carrying a dual amino acid substitution associated with reduced susceptibility to neuraminidase inhibitors
Emi Takashita, Seiichiro Fujisaki, Masaru Yokoyama, Masayuki Shirakura, Kazuya Nakamura, Tomoko Kuwahara, Noriko Kishida, Hironori Sato, Ikuko Doi, Yuji Sato, Shinichi Takao, Yukie Shimazu, Takeshi Shimomura, Takuo Ito, Shinji WatanabeiD, Takato Odagiri, The Influenza Virus Surveillance Group of Japan
Abstract
We detected influenza A(H1N1)pdm09 viruses carrying dual H275Y/I223R, H275Y/I223K, or H275Y/G147R substitutions in their neuraminidase protein, respectively. These viruses showed cross-resistance to oseltamivir and peramivir and reduced susceptibility to zanamivir. The H275Y/G147R virus retained its replication capability at least in vitro, but the H275Y/I223R and H275Y/I223K viruses did not.
Reproducibility ledger
Nobody has reported trying to reproduce this paper yet. Did you replicate an experiment or run the code? Your report saves the next lab a week.
Sign in to file a reproducibility report.
Related papers
- Molecular pathway of influenza pan-neuraminidase inhibitors resistance in an immunocompromised patient
Y. Abed, M. Schibler, … G. Boivin·bioRxiv·Sep 16, 2019
- Increase of permissive secondary antiviral mutations in the evolution of A(H1N1)pdm09 influenza virus neuraminidases
Susanne C. Duwe, Jeanette Milde, … Ralf Dürrwald·VirusesPeer-reviewed·Jul 11, 2024
- Structural insight into the mechanism of neuraminidase inhibitor-resistant mutations in human-infecting H10N8 Influenza A virus
Babayemi O. Oladejo, Yuhai Bi, … Yan Wu·bioRxiv·Jul 26, 2018
- Predicting potentially permissive substitutions that improve the fitness of A(H1N1)pdm09 viruses bearing the H275Y NA substitution
R. Farrukee, V. Gunalan, … A. C. Hurt·bioRxiv·Mar 21, 2021
- Unraveling molecular basis for reduced neuraminidase inhibitors susceptibility in highly pathogenic avian influenza A (H5N1) viruses isolated from chickens in India
- Characterization of influenza B virus variants with reduced neuraminidase inhibitor susceptibility
R. Farrukee, A. E. Zarebski, … A. C. Hurt·bioRxiv·Sep 4, 2018