Jasmonate Primes Plant Responses to Extracellular ATP through Purinoceptor P2K1

Abstract

Extracellular ATP (eATP) signaling in Arabidopsis thaliana is mediated by the purinoceptor P2K1. Previous studies have clarified that the downstream transcriptional responses to eATP involve jasmonate (JA)-based signaling components such as the JA receptor (COI1) and JA-responsive bHLH transcription factors (MYCs). However, the specific contributions of JA signaling itself on eATP signaling are unexplored. Here, we report that JA primes plant responses to eATP through P2K1. Our findings show that JA treatment significantly upregulates P2K1 transcription, corroborating our observation that JA facilitates eATP-induced cytosolic calcium elevation and transcriptional reprogramming in a JA signaling-dependent manner. Additionally, we find that salicylic acid pretreatment represses eATP-induced plant response. These results suggest that JA accumulation during biotic or abiotic stresses may potentiate eATP signaling, enabling plants to better cope with subsequent stress events. SIGNIFICANCE STATEMENTPlant hormone jasmonate (JA) enhances plant responses to extracellular ATP (eATP) in Arabidopsis thaliana through a mechanism dependent on the JA receptor COI1 and the eATP receptor P2K1. The reciprocal amplification of these signals provides a mechanistic explanation for how plants effectively respond to different stress events.