Crebinostat Facilitates Memory Formation

ObjectivesProtein modifications importantly contribute to memory formation. Protein acetylation is a posttranslational modification of proteins that regulates memory formation. Acetylation level is determined by the relative activities of acetylases and deacetylases. Crebinostat is a histone deacetylase inhibitor. In this study, our aim was to examine whether Crebinostat affects memory formation in object recognition task. Further, we aimed to understand regulation of plasticity-related proteins by Crebinostat. MethodsObject recognition task was used to examine the effect of Crebinostat on memory formation. The effect of Crebinostat on plasticity-related proteins was examined using Western blot experiments. ResultsWe show that Crebinostat facilitates memory formation by a weak training in object recognition task. Further, this compound enhances acetylation of -tubulin, and reduces the level of histone deacetylase 6, an -tubulin deacetylase. ConclusionsThe results suggest that enhanced acetylation of -tubulin by Crebinostat contributes to its facilitatory effect on memory formation. AbstractProtein modifications importantly contribute to memory formation. Protein acetylation is a posttranslational modification of proteins that regulates memory formation. Acetylation level is determined by the relative activities of acetylases and deacetylases. Crebinostat is a histone deacetylase inhibitor. Here we show that in object recognition task, Crebinostat facilitates memory formation by a weak training. Further, this compound enhances acetylation of -tubulin, and reduces the level of histone deacetylase 6, an -tubulin deacetylase. The results suggest that enhanced acetylation of -tubulin by Crebinostat contributes to its facilitatory effect on memory formation.