Methods in molecular biologyMolecular BiologyPeer-reviewed journal article
Infrared Spectroscopy for Structure Analysis of Protein Inclusion Bodies
Abstract
Infrared (IR) spectroscopy is a widely used technique for evaluation of protein secondary structure. In this chapter, we focus on the application of this analytical technique for analysis of inclusion bodies. After a general introduction to protein analysis by IR spectroscopy different approaches for spectra acquisition, data processing and secondary structure evaluation are presented.
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