Spectroscopy Techniques in Biomedical and Chemical Research

Arthroscopic Raman Probe Provides Biomarkers of TMJ Tissue Composition that Account for Tissue Function

This study validates arthroscopic Raman spectroscopy for quantifying TMJ tissue composition and mechanical properties, showing strong correlations with indentation and tensile modulus.

Absolute Quantification of Tissue Composition by Raman Spectroscopy

This paper introduces a method to quantify tissue composition using Raman spectroscopy, validated on cartilage with high accuracy compared to biochemical assays.

Amino Acid Remodeling Precedes Malignant Transformation in Oral Epithelial Cells

The paper identifies distinct Raman and proteomic signatures in potentially malignant oral epithelial cells that differentiate them from healthy and malignant cells, highlighting amino acid metabolism changes as early indicators of malignancy.

Label-free identification and classification of leukocyte subpopulations in peripheral blood smears by optical photothermal infrared spectroscopy

This paper uses optical photothermal infrared spectroscopy to classify leukocyte subpopulations in blood smears without staining, achieving accurate separation of granulocytes, lymphocytes, and monocytes.

Coupling Raman Spectroscopy and Acoustic Microscopy to Characterize Glioblastoma Resistance

This study uses Raman spectroscopy and acoustic microscopy to identify biophysical markers of glioblastoma resistance, finding distinct biochemical and mechanical differences between resistant and sensitive cells.

AI-ASSISTED NANOMEDICINE REVEALS SYSTEMIC IMMUNE IMPACT IN OVARIAN CANCER METASTASIS

The paper uses AI-assisted surface-enhanced Raman spectroscopy to analyze immune cell responses to ovarian cancer cells and CSCs, revealing distinct biochemical signatures.

Stainless Steel vs Calcium Fluoride for biomedical Raman Spectroscopy

This study compares calcium fluoride and stainless steel substrates for Raman spectroscopy in diagnosing colonic disease, finding that optimized calcium fluoride matches stainless steel performance but at lower cost.

Amide I kurtosis maps cardiac amyloid in human tissue by ultrabroadband multiplex coherent anti-Stokes Raman scattering (CARS) microscopy

This paper introduces a label-free method using amide I kurtosis from CARS microscopy to detect cardiac amyloid in human tissue, showing high spatial correlation with Congo red staining.

Bone Material Properties Distributions from quantitative Raman analysis: a distribution-based approach to human bone matrix characterization

This paper introduces Bone Material Properties Distributions (BMPDs) to characterize biochemical heterogeneity in human bone matrix using quantitative Raman analysis, showing robustness and reproducibility across tissue processing methods.

Nucleic acid turnover and lipid remodeling distinguish T cell activation and exhaustion states via label-free Raman spectroscopy

This paper uses label-free Raman spectroscopy to distinguish T cell activation and exhaustion states with high accuracy, identifying nucleic acid turnover and lipid remodeling as key features.

Seeing the chemistry of biomolecular condensates: in situ mapping of composition and water content

This paper introduces Raman spectroscopy with spectral phasor analysis to non-invasively map the chemical composition and water content of biomolecular condensates, revealing insights into their physical properties.

Spatial-filtering nanoscopy for 40-nm label-free Raman imaging

This paper introduces spatial-filtering nanoscopy (SFN), a label-free super-resolution Raman imaging technique achieving 40 nm resolution without exogenous labels.

Data-driven spectroscopic dictionaries and detector-calibrated inference for photon-limited Raman hyperspectral imaging of living cells

The paper introduces a data-driven spectroscopic dictionary and detector-calibrated inference framework for photon-limited Raman hyperspectral imaging of living cells, achieving stable spectral decomposition and improved accuracy.

All-Optical Multimodal Mapping of Single Cell Type-Specific Metabolic Activities via REDCAT

REDCAT maps single-cell type-specific metabolic activities in tissues using multimodal all-optical imaging, revealing metabolic specialization and reprogramming in immune and liver tissues.

Label free multimodal optical imaging of metabolic heterogeneity and correlation in aging by integrating SRS, MPF, FLIM, and SHG

The paper introduces MANIFEST, a label-free imaging platform that integrates multiple optical modalities to analyze metabolic heterogeneity and spatial correlations in aging and disease models.

Synchrotron Nano-FTIR Reveals Carbohydrate-Dependent Protein Conformational Changes at Bacterium-Nanoparticle Interfaces

This study uses synchrotron nano-FTIR to show that carbohydrate-coated nanoparticles induce protein conformational changes in E. coli at the nanoscale.

Structural and compositional profiling of individual biomolecular assemblies by infrared absorbance-modulated evanescent scattering

This paper introduces IR-AMES, a technique for ultrasensitive structural and compositional analysis of individual biomolecular assemblies in aqueous environments, revealing insights into tau oligomers and their interactions with membranes.

Label-free biochemical imaging and timepoint analysis of neural organoids via deep learning-enhanced Raman microspectroscopy

This paper develops a label-free imaging platform using Raman microspectroscopy and deep learning to analyze neural organoids, revealing spatiotemporal biochemical changes.

DNA fragment length analysis using machine learning assisted vibrational spectroscopy

This paper presents a machine learning-assisted vibrational spectroscopy method for rapid, label-free DNA fragment length analysis with high accuracy.

Deep-ultraviolet microscopy reveals biomolecular spatiotemporal intracellular dynamics

This study uses deep-ultraviolet microscopy to analyze intracellular dynamics with biomolecular specificity, revealing differences in metabolic and organelle activity across prostate cancer cell lines.

Broadband electronic resonance coherent anti-Stokes/Stokes Raman scattering microscopy

This paper introduces BER-CARS/CSRS microscopy, which enables highly sensitive, label-free imaging of endogenous chromophores with broad spectral coverage, applied to living cells and tissues.

Evaluating Limits of Machine Learning-Assisted Raman Spectroscopy in Classification of Biological Samples

This study evaluates how experimental factors affect ML-assisted Raman spectroscopy classification accuracy, finding that data quality and spectral similarity are key limitations.

DNA methylation and hydroxymethylation quantification using vibrational spectroscopy

This paper demonstrates that ATR-FTIR spectroscopy with regression models can rapidly and non-destructively quantify DNA methylation and hydroxymethylation in DNA samples, including circulating tumor DNA.

Instantaneous Phase-Shifting Optothermal Microscopy for Live-cell Metabolic Monitoring

This paper introduces IPSOM, a fast optothermal microscopy method for live-cell metabolic monitoring, demonstrating its application in tracking lipid remodeling in adipocytes.

Dynamic, single-cell monitoring of CAR T cell identity and activation with Raman spectroscopy

Gold-nanorod surface-enhanced Raman spectroscopy with LightGBM separates CAR T from untransduced T cells, tracks antigen-specific activation dynamics, and discriminates sorted CAR+ from CAR- patient T cells.

Optical photothermal infrared imaging of fatty acid esterification in the ER of living cells

The paper uses optical photothermal infrared imaging to study palmitic acid metabolism in live cells, revealing diacylglycerol intermediates and gel phase changes in the ER.

PLANCK: super-multiplex optical imaging without labeling

This paper introduces PLANCK, a label-free super-multiplex optical imaging technique that uses AI to decode vibrational images into molecular species, achieving high specificity and scalability.

A Raman Spectroscopy-Based Method for Label-Free Discrimination of Human Inhibin α, Inhibin B, and Activin A

This study uses Raman spectroscopy to distinguish human Inhibin α, Inhibin B, and Activin A based on their vibrational fingerprints.

In Vitro Detection of Breast Cancer Cell Types Using Machine Learning-Assisted Spectral Fingerprinting of SWCNTs

This paper uses machine learning and SWCNT spectral fingerprinting to detect and subtype breast cancer cells in live culture with high accuracy.

Hydration and H/D exchange-dependent infrared signatures of the GCN4 leucine zipper

This study uses vacuum-dried ATR-FTIR spectroscopy to analyze the structural features and HDX kinetics of GCN4, revealing distinct Amide I bands in dry samples.