Phytochemical Analysis And Pharmacological Effects Of Gongronema Latifolium Extracts On Tamoxifen-Induced Toxicity In Wistar Rats

Abstract

BackgroundTamoxifen, a widely used anti-estrogen medication, is known to induce oxidative stress, hepatic toxicity, and hematological disorders. Gongronem alatifolium, a tropical plant, has been traditionally used in folk medicine to treat various ailments. This study aimed to investigate the phytochemical analysis and pharmacological effects of Gongronema latifolium extracts on tamoxifen-induced toxicity in rats. MethodsThe study employed a randomized controlled trial design, where rats were divided into six groups of six (6) rats each. Group one were administered with distilled water (1 mL), group two tamoxifen-treated (20 mg/kg), group three tamoxifen + zinc sulfate (100 mg/kg), group four tamoxifen + Gongronema latifolium methanol extract (200 mg/kg), group five tamoxifen + Gongronema latifolium ethanol extract (200 mg/kg), and group six tamoxifen + zinc sulfate + Gongronema latifolium methanol extract 100 mg/kg + 200 mg/kg. Phytochemical analysis was conducted using standard methods. Oxidative stress markers, biochemical parameters, and hematological parameters were measured using standard assays. ResultsThe results of the study revealed significant reduction in MDA levels and improved SOD activities, increases in the total protein and reduced ALT and AST activities, reduced total cholesterol and triglycerides levels, but increases HDL level, reduced creatinine and urea levels, while RBC and Hb levels were significantly improved. Conclusion: This study provides new insights into the pharmacological effects of Gongronema latifolium extracts and highlights their potential as a natural remedy for reducing tamoxifen toxicity. The studys findings have significant implications for the development of novel therapeutic strategies for reducing tamoxifen toxicity.